Improved Methods for Infection of Plant Protoplasts with Viral Ribonucleic Acid
نویسندگان
چکیده
Protoplasts, RNA, Plant Viruses, Tobacco Mosaic Virus, Polyethylene Glycol Infection of tobacco protoplasts with R N A of tobacco mosaic virus and the bromoviruses has been studied. The quality of the R N A was very important. Ribonuclease activity was controlled by incorporating 5 mM ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus R N A but infection with TM V R N A was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral R N A examined.
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